| Description
|
Biotin-20-dUTP, lyophilized powder (Biotin-20-2’-deoxyuridine-5’-triphosphate, tetralithium salt) |
| Category
|
Modified nucleotides |
| Shipping temperature
|
2°C to 8°C |
| Shipping conditions
|
blue ice |
| Storage
|
-30°C to -2°C |
| Hazard pictogram
|
n/a |
| Signal word
|
n/a |
| Hazard statements
|
n/a |
| Properties
|
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. |
| Conjugation
|
Biotinylated |
| About
|
The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen. |