| Description
|
Flow Cytometry Fixation/Permeabilization Kit |
| Category
|
Accessory reag - Cell Bio |
| Shipping temperature
|
15°C to 30°C |
| Shipping conditions
|
n/a |
| Storage
|
2°C to 8°C |
| Hazard pictogram
|
GHS05 corrosive, GHS06 toxic, GHS08 systemic health hazards |
| Signal word
|
Danger |
| Hazard statements
|
H227 Combustible liquid H301 + H311 + H331 Toxic if swallowed, in contact with skin or if inhaled H314 Causes severe skin burns and eye damage. |
| Additional description
|
FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. |
| Test
|
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. |