| Description
|
PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx |
| Category
|
PCR and related reagents |
| Shipping temperature
|
2°C to 8°C |
| Shipping conditions
|
blue ice |
| Storage
|
-30°C to -2°C |
| Hazard pictogram
|
N/A |
| Signal word
|
N/A |
| Hazard statements
|
N/A |
| Disease
|
Listeria monocytogenes and Listeria species are gram-positive, rod-shaped, facultatively anaerobic, and non spore-forming. The major human pathogen in the Listeria genus is L. monocytogenes. It is usually the causative agent of the relatively rare bacterial disease listeriotic, listeriosis a serious infection caused by eating food contaminated with the bacteria. The disease affects pregnant women, newborns, adults with weakened immune systems, and the elderly. Antibodies and ELISA test kits for detection of listeria antigens. |
| Group
|
PCR, polymerase chain reaction |
| About
|
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers. |
| Properties
|
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. |
| Additional description
|
Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes. |